Quantitative Analysis of Antioxidant Biomarkers in Cucumis Melo Var. Agrestis Leaves Using a Validated Hptlc Technique
Keywords:
Bioflavonoids, Rutin, Gallic acid, Quercetin, Antioxidant and Developed method of HPTLCAbstract
Background: This study focused on using the HPTLC method for the simultaneous estimation of natural antioxidant markers, specifically rutin, gallic acid, and quercetin, from the plant extract of Cucumis melo var. agrestis (CME).
Aims and Objectives: The primary objective of this research was to extract and simultaneously quantify the three antioxidant markers— rutin, gallic acid, and quercetin—from CME using normal phase HPTLC.
Materials and Methods: Compounds were separated on TLC aluminum plates precoated with silica 60 F 260, followed by the detection of rutin, gallic acid, and quercetin. Densitometric scanning at 250 nm was performed using a Camag TLC scanner 3 equipped with winCATS software. This method was further validated for linearity, precision, accuracy, and sensitivity according to ICH guidelines.
Results: The calibration plots showed a strong linear relationship with R2 values of 0.982, 0.9892, and 0.9860 for rutin, gallic acid, and quercetin, respectively. Accuracy was assessed through recovery studies at three different levels, yielding an average recovery rate between 95% and 98% for all three markers.
Conclusion: The developed HPTLC method is suitable for detecting antioxidant markers in botanicals and herbal formulations.
How to cite this article:
Quantitative Analysis of Antioxidant Biomarkers
in Cucumis Melo Var. Agrestis Leaves Using a
Validated Hptlc Technique. J Adv Res Biochem
Pharma 2024; 7(1): 30-0.
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