Assessment of Antibacterial Activity and Phytochemistry of Different Clones of Tea Grown and Processed at NTHRI

Authors

  • Madiha Bashir National Tea and High Value Crops Research Institute, Shinkiari, Mansehra, Pakistan.
  • Farrukh Siyar Hamid National Tea and High Value Crops Research Institute, Shinkiari, Mansehra, Pakistan.
  • Abdul Waheed National Tea and High Value Crops Research Institute, Shinkiari, Mansehra, Pakistan.
  • Naveed Ahmed National Tea and High Value Crops Research Institute, Shinkiari, Mansehra, Pakistan.
  • Seemab Ali National Tea and High Value Crops Research Institute, Shinkiari, Mansehra, Pakistan.
  • Hussain Shah Plant Sciences Division, Pakistan Agricultural Research Council, Islamabad, Pakistan.
  • Nadia Khan National Tea and High Value Crops Research Institute, Shinkiari, Mansehra, Pakistan.
  • Fayaz Ahmad National Tea and High Value Crops Research Institute, Shinkiari, Mansehra, Pakistan.
  • Saqib Mumtaz Department of Biosciences, COMSATS, Islamabad, Pakistan.
  • Sohail Aslam National Tea and High Value Crops Research Institute, Shinkiari, Mansehra, Pakistan.

Abstract

Background and Objective: Medicinal plants have been a major source of therapeutic agents to cure diseases. The present study was aimed to study phytochemistry and antimicrobial potential of tea clones i.e P3, P5, and P7 grown and processed in Black tea at NTHRI.

Materials and methods: Various analytical techniques were applied for phytochemical analysis to identify phytocompounds. The phytochemical analysis showed the presence of alkaloids, flavonoids, phlobatannins, glycosides, carotenoids, saponins, phenols, and terpenoids by changing the color of the medium when treated with respective reagents in aqueous extracts.

Results: Phlobatannins and steroids were found absent in all varieties in ethanolic extracts while seen positive in all varieties in aqueous extracts. The Minimum Inhibitory Concentration (MIC) for antimicrobial activity was determined by the agar well diffusion method against two bacterial strains Staphylococcus aureus and Salmonella typhi. P3 showed highest antimicrobial activity against both bacterial strains i.e 15.1±0.84 in 50 ppm and 17.3±0.62 in 100 ppm against S. aureus, while 7.9±0.32 in 50 ppm and 10.06±0.6 in 100 ppm against S. typhi. P7 also showed maximum activity against both strains i.e 13.3±1.53 in 50 ppm, 15.02±0.96 in 100 ppm against S. aureus and 6.16±0.76 n 50 ppm, 9.23±0.68 in 100 ppm against S. typhi and the low activity against both strains were observed in P5 i.e. 4.9±1.10 in 50 ppm, 5.6±0.36 in 100 ppm against S. aureus and 6.16±0.76 in 50 ppm 9.23±0.68 in 100 ppm against S. typhi.

Conclusion: Our findings suggest that consumption of these varieties could be valuable to promote consumer health and also serve as a good source of energy and nutrients.

How to cite this article: Bashir M, Hamid FS, Waheed A. Assessment of Antibacterial Activity and Phytochemistry of Different Clones of Tea Grown and Processed at NTHRI. Int J Adv Res Med Chem 2020; 2(1): 5-10.

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Published

2020-05-15