Assessment of Microbial Characteristics of Processed Palm Weevil “Rhynchophorus phoenicis” Larvae Sold in some Markets Area in Bayelsa State, Nigeria
Keywords:
Contaminants, Food, Microorganisms, Public Health, Rhynchophorus phoenicisAbstract
Problem Statement: The larvae of palm weevil (Rhynchophorus phoenicis) is commonly processed and consumed as food in the Niger Delta region of Nigeria especially in the rural areas.
Aims and Objective: This study investigated the microbial characteristics of processed larvae of Rhynchophorus phoenicis sold in some market area in Yenagoa metropolis, Bayelsa State, Nigeria.
Methods: Triplicate samples of processed Rhynchophorus phoenicis larvae were obtained from vendors in Opolo and Tombia market area in Yenagoa metropolis, Nigeria. The samples were analyzed following standard microbiological procedures.
Result: Results showed that the total heterotrophic bacteria, Enterobacteriaceae bacteria and total fungi counts ranged from 0.85-8.6 x 105 cfu/g, 1.18-8.73 x 102 cfu/g and 1.52-7.97 x 103 cfu/g, respectively. There was statistical deviation (P<0.05) among the Rhynchophorus phoenicis samples. The microbial isolates found in the samples were Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Bacillus, Proteus and Micrococcus species (bacteria), Aspergillus niger, Aspergillus flavus, Penicillium and Rhizopus species (fungi). The similarity in interaction of the microbial diversity between samples from each vendor ranged from 57.14-82.35% for Sorenson qualitative index and 0.40-0.70 (which is equivalent to 40-70%) for Jaccard index. The similarity is above a critical level of significance of 0.5 or 50% except for samples from vendor A - C using Jaccard index.
Conclusion: Most of the microorganisms isolated especially the fungi could produce toxins and are pathogenic, hence, there is a need to frequently monitor the processed Rhynchophorus phoenicis larvae sold in the study area to ascertain its acceptability with respect to microbial quality.
How to cite this article:
Seiyaboh EI, Izah SC. Assessment of Microbial Characteristics of Processed Palm Weevil “Rhynchophorus phoenicis” Larvae Sold in some Markets Area in Bayelsa State, Nigeria. J Adv Res Med Sci Tech 2020; 7(2): 1-6.
DOI: https://doi.org/10.24321/2394.6539.202004
References
Okunowo WO, Olagboye AM, Afolabi LO, Oyedeji AO (2017). Nutritional Value of Rhynchophorus phoenicis (F.) Larvae, an Edible Insect in Nigeria. African Entomology, 25(1): 156–163. doi:10.4001/003.025.0156.
Assielou B, Due EA, Koffi MD, Dabonne S, Kouame PL (2015). Oryctes owariensis Larvae as Good Alternative Protein Source: Nutritional and Functional Properties. Annual Research & Review in Biology, 8(3): 1-9.
Thomas CN, Briyai FO (2019). Traditional Consumption of Rhynchophorus phoenicis Larvae (F.) as Human Food in Niger Delta Area, Nigeria. Journal of Biology and Genetic Research, 5 (1): 39 – 45.
Gnanda EP, Mauricette QS (2018). Biological studies on palm tree weevil Rhynchophorus phoenicis fabricius (Coleoptera; Curculionidae): An interest food bug in Côte d’Ivoire (West Africa). International Journal of Biosciences, 13(1): 137-147.
Okore O, Avaoja D, Nwana I (2014). Edible Insects of the Niger Delta Area in Nigeria. Journal of Natural Sciences Research, 4(5): 1- 9.
Izah SC, Angaye CN, Aigberua AO, Nduka JO (2017). Uncontrolled bush burning in the Niger Delta region of Nigeria: potential causes and impacts on biodiversity. International Journal of Molecular Ecology and Conservation, 7(1): 1-15
Ukoroije RB, Bobmanuel RB (2019). A call for domestication and mass rearing of Oryctes owariensis larvae (Coleoptera: Scarabaeidae). Noble International Journal of Scientific Research, 03(10): 103-108.
Okoli IC, Olodi WB, Ogbuewu IP, Aladi NO, Okoli CG (2019). Nutrient composition of African palm grub (Rhynchophorus phoenicis) larvae harvested from raphia palm trunk in the Niger-Delta swamps of Nigeria. Asian Journal Biological Science, 12: 284-290.
Umedum CU, Ofoma C, Ekwudu O, Nwabunnia E (2011). Microbial Quality of Roasted Larvae of the Palm Weevil 'Rhyncophorus phoenicis' Sold at Okija and Oba Junctions, Anambra State, Nigeria. Journal of Medical Laboratory Science, 20(1): https://www.journalquality.info/jpps-criteria/inactive-title (assessed: 2019-03-01)].
Ebenebe CI, Okpoko VO (2015). Microbiological quality of raw and roasted African palm weevil (Rhynchophorus phoenicis) consumed in the south Eastern Nigeria. Animal Research International, 12(2): 2159 – 2165
Onyeike EN, Ayalogu EO, Okaraonye CC (2005). Nutritive value of the larvae of raphia palm beetle (Oryctes rhinoceros) and weevil (Rhyncophorus pheonicis). Journal of the Science of Food and Agriculture, 85(11): 1822–1828. doi:10.1002/jsfa.2054
Izah SC, Aseiba ER, Orutugu LA (2015). "Microbial quality of polythene packaged sliced fruits sold in major markets of Yenagoa Metropolis, Nigeria. Point Journal of Botany and Microbiology Research, 1(3): 30 – 36.
Pepper IL, Gerba CP (2005). Environmental microbiology. A laboratory manual. Second edition. Elsevier academic press.
Benson HJ (2002) Microbiological Applications: Laboratory Manual in General Microbiology/complete version, 5th edition. McGaraw-Hill, New York.
Cheesbrough M (2009). District Laboratory Practice in Tropical Countries. Low price Edition part 2. Cambridge press, England.
Holt JG, Kneg NR, Sneath PHA, Stanley JT, Williams ST (1994). Bergey’s Manual of Determinative Bacteriology. William and Wilkins Publisher, Maryland. New York.
Ellis D, Davis S, Alexiou H, Handke R, Bartley R (2007). Descriptions of Medical Fungi. Second Edition. Printed in Adelaide by Nexus Print Solutions, Underdale, South Australia.
Ohimain EI, Daokoru-Olukole C, Izah SC, Eke RA, Okonkwo AC (2012). Microbiology of palm oil mill effluents. Journal of Microbiology and Biotechnology Research 2(6):852 – 857.
Izah SC, Ohimain EI, Jenakumo N (2013). Physicochemical and microbial screening of palm oil mill effluents for amylase production. Greener Journal of Biological Sciences. 3(8): 314 – 325.
Izah SC, Ohimain EI (2013). Microbiological quality of crude palm oil produced by smallholder processors in the Niger Delta, Nigeria. Journal of Microbiology and Biotechnology Research, 3(2): 30 – 36.
Ogbeibu AE (2014). Biostatistics. A practical approach to research and data handling. 2nd edition, Mindex Publishing Company Limited, Benin-City Nigeria.
International Commission on Microbiological Specifications for Foods (ICMSF) (1996). Microorganisms in Foods 5: Microbiological Specifications of Pathogens.
Ineyougha ER, Orutugu LA, Izah SC (2015). Assessment of Microbial Quality of Smoked Trachurus trachurus sold in some Markets of Three South-South States of Nigeria. International Journal of Food Research, 2: 16 – 23.
Bush LM, Perez MT (2014). Pseudomonas and Related Infections. The merck manual, Professional Edition. http://www.merckmanuals.com/professional/infectious_diseases/gram-negative_bacilli/pseudomonas_and_related_infections.html. Accessed 26th March, 2015.
Odu NN, Imaku LN (2013). Assessment of the Microbiological Quality of Street-vended Ready-To-Eat Bole (roasted plantain) Fish (Trachurus Trachurus) in Port Harcourt Metropolis, Nigeria. Researcher, 5(3):9-18.
Kigigha LT, Berezi J, Izah SC (2017). Bacteriological Quality Assessment of Meat Pie Sold in Yenagoa Metropolis, Nigeria. EC Nutrition, 6(6): 189-195.
Orutugu LA, Izah SC, Aseibai ER (2015). Microbiological quality of Kunu drink sold in some major markets of Yenagoa Metropolis, Nigeria. Continental Journal of Biomedical Science. 9(1): 9 – 16.
Kigigha LT, Samson GA, Izah SC, Aseibai ER (2018) Microbial Assessment of Zobo Drink Sold in Some Locations in Yenagoa Metropolis, Nigeria. EC Nutrition 13(7): 470-476.
Kigigha LT, Izah SC, Kpea TB (2015). Microbiological quality of fermented Cassava Flakes (Gari) sold in Yenagoa, Metropolis, Nigeria. Bulletin of Advanced Scientific Research, 01 [07]: 157 – 160
Kigigha LT, Ovunda HO, Izah SC (2015). Microbiological quality assessment of suya sold in Yenagoa Metropolis, Nigeria. Journal of Advances in Biological and Basic Research 01[05]: 106 – 109
Hashem M (2011). Isolation of Mycotoxin-producing Fungi from Fishes Growing in Aquacultures. Research Journal of Microbiology, 6: 862-872.
Pratiwi C, Rahayu WP, Lioe HN, Herawati D, Broto W, Ambarwati S (2015). The effect of temperature and relative humidity for Aspergillus flavus BIO 2237 growth and aflatoxin production on soybeans. International Food Research Journal, 22(1): 82-87.
Wild CP, Gong YY (2010). Mycotoxins and human disease: a largely ignored global health issue. Carcinogenesis, 31:71-82.
Akinyemi AA, Adejola AQ, Obasa SO, Ezeri GNO (2011). Aflatoxins in Smoked?dried Fish sold in Abeokuta, Ogun State, South?west Nigeria. Proceedings of the Environmental Management Conference, Federal University of Agriculture, Abeokuta, Nigeria. pp. 478 – 487.
Cheikyula TJ, Awobode HO (2014). Microbial Flora and Nutrient Content of Market Bought Smoked African Cat Fish Clarias gariepinus from Jos, Nigeria. Food Science and Quality Management, 32:34 – 40.
Izah SC, Ohimain EI (2016). Microbiological quality of palm oil used in Nigeria: Health impacts perspective. Point Journal of Botany and Microbiology Research, 2(1): 037 – 045.
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