Evaluation of Secondary Metabolites and Proximate Composition in Different Varieties of Green Tea
Abstract
Leaves of Camellia sinensis are composed of a variety of secondary metabolites play a vital role in human nutrition and health. In this study three different varieties of processed green tea includes P3, P5 and P9 were collected from National Tea and High Value Crops Research Institute (NTHRI), Shinkiari, Mansehra, Pakistan for proximate composition analysis and secondary metabolites analysis. Standard methods were carried out to evaluate proximate composition analysis of crude fat, crude fiber, ash content and moisture content in all tea samples. Presence of secondary metabolites in aquaeous and ethanolic tea leaves extracts was recognized by its colour intensity using standard chemical tests.. Composition of moisture content, ash content, crude fiber and crude fat in tea samples were range between 3.2% to 6.9%, 6% to 5.4%, 10.4% to 15.6%, 3.5% to 10.1% respectively. Secondary metabolites like alkaloids, saponins and flavonoids were found positive in aqueous extract but absent in ethanolic extract of all samples while phlobatannin and carotenoids were found absent in both tea extracts.
How to cite this article: Bashir A, Waheed A, Hamid FS et al. Evaluation of Secondary Metabolites and Proximate Composition in Different Varieties of Green Tea. J Adv Res Biochem Pharma 2019; 2(2): 14-19.
References
AACC. Approved methods of analysis. 10th Ed.The American association of cereal chemists. St. Paul, MN. 2000.
Akhlas M, Ahmed T, Siyar HF et al. Qualitative assessment of fresh tea produced in Pakistan growing under different agro-ecological conditions and fertilizer treatments. Pak J Bot 2003; 35: 779-790.
Dawodu MO, Obimakinde O, Samuel O et al. Trace metal concentrations in some tea leaves consumed in Ibadan, Nigeria. Afri J Agri Res 2013; 8(46): 5771-5775.
Edeogal HO, Okwu DE, Mbaebial BO et al. Phytochemical constituents of some Nigerian medicinal plant. Afri J Biotech 2005; 4(7): 685-688.
Han LX, Li R. Determination of minerals and trace elements in various tea by ICP-AES, Spectr. Spectral Anal. 2002; 22: 304-306
Ruan JY. Effect of processing on the Pb and Cu pollution of tea in Chinese. J Tea Sci 2006; 26: 95-101.
Han WY, Liang YR, Yang YJ et al. Effect of processing on the Pb and Cu pollution of tea in Chinese. J Tea Sci 2006; 26: 95-101.
Harbone J. Experimental methods in analytical chemistry. New York, Chapman and Hall. 1973; 140-145.
Idu AS, Igeleke J. Preliminary phytochemical analysis of some plant seeds. Res J Chem Sci 2012; 1(3).
Iqbal HZ. Mineral constituents of tea (camellia sinensis L) Department of agricultural chemistry faculty of nutrition of sciences. 2002.
Ismail ME. Manickam AM, Danial A. Rahmat and A. Yahaya. Chemical composition and antioxidant activity of Strobilanthes crispus leaf extract. J Nutr Biochem 2000; 11(11): 536-542.
Kaur HP, Kaur S, Rana S. Antibacterial Activity and Phytochemical Profile of Green Tea, Black Tea and Divya Peya Herbal Tea. Int J Pure App Biosci 2015; 3(3): 117-123.
Kurma A, Nair AGC, Reddy AVR et al. Availability of essential elements in Indian and the United States tea brands. Fd Chem 2005; 89: 441-448.
Latif A, Jan AU, Chishti AF et al. Assessing potential of local tea production in Pakistan. Sarhad J Agric 2008; 24(2): 340-343.
McKay DL, Blumberg JB. The role of tea in human health: An update. J Am Coll Nutr 2002; 21: 1-13.
Noroozi M, Angerson WJ, Lean MEJ. Effects of flavonoids and vitamin c on oxidative DNA damage to human lymphocytes. Am J Clin Nutr 67: 1210-1218.
Okwu DE. International Journal of Molecular Medicine and Advances in Science 2005; 22: 199-203.
Milani RF, Morgano MA, Saron ES et al. Evaluation of Direct Analysis for Trace Elements in geen Tea and Herbal Beverages by ICP-MS. J Braz Chem Soc 2015; 26(6): 1211-1217.
Rehman SU, Almas K, Shahzadi N et al. Effect of time and temperature on infusion of tannins from commercial brands of tea. Int J Agric Biol 2002; 4(2): 285-287.
Sartippour MR, Shao ZM, Heber D et al. Green tea inhibits vascular endothelial growth factor (VEGF) induction in human breast cancer cells. J Nutr 2002; 132: 2307-2311.
Sato T, Miyata G. The nutraceutical benefit, part I: green tea. Nutrition 2000; 16: 315-317.
Sofowora A. Medicinal plants and Traditional medicine in Africa: spectrum books Ltd, Ibadan, Ibadan, Nigeria. 1993; 289.
Wei X, Chen M, Xiao J et al. Composition and bioactivity of tea flower polysaccharides obtained by different methods. Carbohydr Poly 2010; 79(2): 418-422.
Weisburger JH. Approaches for chronic disease prevention based on current understanding of underlying mechanisms. Am J Clin Nutr 2000; 71(6): 1710S-1714S.
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