Blood Glucose, some Electrolytes Levels and Stress Oxidative Status of Female Hyperthyroid Patients under Treatment

Authors

  • Derouiche Samir Department of Cellular and Molecular Biology, Faculty of natural science and life, University of El-oued, El-oued 39000,Algeria
  • Djouadi Anfal Department of Cellular and Molecular Biology, Faculty of natural science and life, University of El-oued, El-oued 39000, Algeria.
  • Belimi Naima Department of Cellular and Molecular Biology, Faculty of natural science and life, University of El-oued, El-oued 39000, Algeria.
  • Louetri Khadidja Department of Cellular and Molecular Biology, Faculty of natural science and life, University of El-oued, El-oued 39000, Algeria.
  • Hachefa Salima Department of Cellular and Molecular Biology, Faculty of natural science and life, University of El-oued, El-oued 39000, Algeria.

Keywords:

Hyperthyroidism, Stress oxidative, Electrolytes, Sodium

Abstract

The present study was designed to investigate the hyperthyroidism effects on stress oxidative state, blood
glucose, hemoglobinand serum electrolytes levels in female patients under treatment. In this work 24 cases of
overt hyperthyroidism were chosen, 24 age matched controls were chosen. Blood samples were collected from
them and T3, T4 and TSH levels were measured. Also, glucose, hemoglobin, MDA, GSH, Sodium, Potassium
and Chloride levels in blood was measured. Results obtained show that Lipid peroxidation (p <0.01), blood
glucose (p<0.05) and hemoglobin (p<0.001) level were significantly elevated simultaneously with a significant
decrease (p <0.05) in the levels of GSH in all patients with thyroid dysfunction as compared to control. The
levels of serum sodium was significantly increased (p <0.05)in these cases than controls and on the other
hand serum potassium and chloride concentrationswere significantly and non-significantly decreased
successively in hyperthyroid patients when compared to the control subjects. In hyperthyroidismpatients,
there was a significant negative correlation (r= - 0.998; P<0.05) between the levels of reduced glutathione
(GSH) with concomitant increase in MDA levels butthere was a no significant correlation (P>0.05) between
serum MDA values and serum electrolytes and TSH levels.The results of this study reveal the importance
of monitoring the levels of thosestress oxidative levels and biochemicalparameters in thyroid dysfunction
patients before therapy, especially when the disease ismore severe.

References

1. Arvind, B., Shailaza, S., Rahul, R., Mukesh, K.S.
Assessment of serum minerals and electrolytes in
thyroid patients. International Journal of Advances in
Scientific Research 2015; 1(06): 259-263.
2. Derouiche S, Djermoun M, Abbas K. Beneficial Effect
of Zinc on diabetes induced kidney damage and liver
stress oxidative in rats. J. Adv. Biol. 2017;10(1): 2050-
5055.
3. Katarzyna, K.V., Krystyna, O., Eugene, J. K., Czeslaw,
M., Katarzyna, W.S,. Ann, K. Free radical activity and
antioxidant defense mechanisms in patients with
hyperthyroidism due to Graves’ disease during therapy.
Clinica Chimica Acta. 2000; 300 (2):107–117.
4. Samir Derouiche, Anfal Djouadi. An evaluation of stress
oxidative and serum electrolytes in female hypothyroid
patients. Int J Biol Med Res ; 8(1): 5861-5865, 2017
5. Yilmaz, S., Ozan, S., Benzer, F., Canatan, H. Oxidative
damage and antioxidant enzyme activities in
experimental hypothyroidism. Cell BiochemFunct.
2003; 21(4): 325-30.
6. Sarwarm ,A.T. Dietary electrolyte balance: implications
in heat stressed broilers. World’s Poultry Science Journal
2006; 62:638-653.
7. Liamis, G., KalogirouM., Saugos, V., Elisaf, M. Therapeutic
approach in patients with dysnatraemias.Nephrol Dial
Transplant 2006; 21(6): 1564-1569.
8. Schwarza, C., Benedikt, L.A., Arampatzisc,
Spiros.,Fiedlerb G.M., Immermannd, H., Exadaktlyosd,
A.K., Lindnerd, G. Thyroid function and serum
electrolytes:does an association really exist. Swiss
Med Wkly. 2012;142:13669-13675.
9. Murgod, R., Soans, G. Changes in Electrolyte and Lipid
profile in Hypothyroidism. International Journal of Life
science and Pharma research. 2012; 2(3): 185-194.
[10] Beigi I.F., Edelman, I.S. The mechanism of the
calorigenic effect of thyroid hormone Stimulation of
Na+ + K+ activated adenosinetri phosphatase activity.
J gen Physiol.1971; 57: 710
10. Sataline , L.R., Charles, M.D., Powell, M.D., Hamwi ,G.J.
Suppression of the Hypercalcemia of Thyrotoxicosis
by Corticosteroids. N Engl J Med 1962; 267:646-650
11. Vasudevan, N., Ogawa S., Praff, D. Estrogen and
thyroid hormone interactions: Physiological stability
by molecular specificity. Physiol Rev. 2002; 82: 923-944.
12. Buege, J.A., Aust, S.D. Microsomal lipid, Peroxidation.
In:Flesicher, S., Packer, L. (Eds.), Methods in Enzymology.
Academic Press, New-York, 1978; 52: 302–310.
13. Akerboom, T.P.M., Sies, H.. Assay of glutathione,
glutathione disulfide and glutathione mixed disulfides
in biological samples. Methodsenzymol. 1981; 77:
373-382.
14. Blandine Comte, Hubert Vidal, Martine Laville,
Jean-Paul Riou. Influence of thyroid hormones on
gluconeogenesis from glycerol in rat hepatocytes: A
dose-response study. 1990; 39(3): 259–263
15. Elliott S. Erythropoiesis-stimulating agents and other
methods to enhance oxygen transport. Br J Pharmacol.
2008 Jun; 154(3): 529–541. doi: 10.1038/bjp.2008.89
16. DorgalalehA, MahmoodiM, VarmaghaniB, Kiani
nodeF, Saeeidi KiaO, AlizadehSh, TabibianSh, Bamedi
T, MomeniM, AbbasianS, and KashaniKhatibZ. Effect
of Thyroid Dysfunctions on Blood Cell Count and RedBlood Cell Indice. Iran J PedHematol Oncol. 2013;
3(2): 73–77.
17. Derouiche S, DoudiDalal, Atia N. Study of Oxidative
Stress during Pregnancy. Glob J Pharmaceu Sci 4(5):
GJPPS.MS.ID.555646 (2018)
18. DerouicheS, Abbas K ,Djermoune M. Changes in
metabolism of Zinc andcarbohydrate and testis
oxidative stress of diabetic rats fed zinc-over dose
diet. Int J Biol Med Res. 2017; 8(3):6041-6045,
19. Derouiche, S., Abbas, K., Djermoune, M., Ben Amara,
S., Kechrid, Z. The effects of copper supplement on
zinc status, enzymes of zinc activities and antioxidant
status in alloxan- induced diabetic rats fed on zinc
over-dose diet. International Journal of Nutrition and
Metabolism 2013; 5(5): 82-87.
20. Dipak, K.S., Anita, R., Shravani, B., Chainy, G.B.N.
Experimental hyperthyroidism-induced oxidative stress
and impairment of antioxidant defence system in rat
testis. Indian Journal of Expenmental Biology 2005;
43:1058-1067.
21. Samir D and Zine K. influence of calcium supplements
on zinc status, carbohydrate metabolism and the liver
activity of detoxifying glutathione enzymatic system in
alloxan-induced diabetic rats. Journal of Experimental
Biology and Agricultural Sciences, 2013; 1(6):424-429.
22. Kandasamy, B., Jayaraaj I.A, Prabhakar, J. Effect
of Abnormal thyroid hormone changes in lipid
peroxidation and Antioxidantimbalance in Hypothyroid
and Hyperthyroid patients. Int J Biol Med Res. 2011;
2(4): 1122-1126.
23. Venditti, P., Di Meo, S. Thyroid hormone-induced
oxidative stress. Cellular and Molecular Life Sciences.
2006;.63(4): 414-434.
24. Oppenheimer, J.H., Schwartz,H.L. Strait,K.A. Thyroid
hormone action: the plot thickens. European Journal
of Endocrinology. 1994; 130(1): 15-24.
25. Antonio, M., Roberto, F., Andrea S., Nicola N., Vincenzo,
D.,Giuseppe, L., Alfredo, P.i, Elisabetta, M. Hormonal
Regulation of Total Antioxidant Capacity in Seminal
Plasma. J Androl 2009; 30( 5): 534–540.
26. Willcox JK, Ash SL, CatignaniGL .Antioxidants and
prevention of chronicdisease.Crit. Rev Food Sci
Nutr.2004; 44: 275-295.
27. AdelheidWeidinger and Andrey V. Kozlov. Biological
Activities of Reactive Oxygen and Nitrogen Species:
Oxidative Stress versus Signal Transduction.
Biomolecules 2015, 5, 472-484; doi:10.3390/
biom5020472
28. Ersan S, Bakir S, Ersan EE, Dogan O . Examination of
freeradicalmetabolism and antioxidant defense system
elements inpatients withobsessive-compulsive disorder.
Prog. Neuropsychopharmacol. Biol.Psychiatry.2006; 30:
1039-42.
29. Beigi, I.F., Edelman, I.S. The mechanism of the
calorigenic effect of thyroid hormone: stimulation of
Na+ + K+ activated adenosinetriphosphatase activity.
J gen Physiol. 1971; 57: 710.
30. Ornellas, S.D., Grozovsky, R., Goldenberg, R.C.,
Carvalho, D.P., Fongi. P., Gugginoi, W.P., Morales M.
Thyroid hormone modulates ClC-2 chloride channel
gene expression in rat renal proximal tubules. J of
Endocrin.2003; 178: 503–511.
31. Schrier, R.W. Body fluid volume regulation in health
and disease: A unifying hypothesis. Ann. Intern. Med.
1990; 113: 155–159.
32. Tripathi, U., Deepak, N. The plant extracts of
Momordicacharantia and Trigonellafoenumgraecum
have antioxidant and anti-hyperglycemic properties
for cardiac tissue during diabetes mellitus. Oxid. Med.
Cell. Longev. 20092; (5): 290-296.

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Published

2018-09-07

Issue

Vol. 1 No. 1&2 (2018): Journal of Advanced Research in BioChemistry and Pharmacology

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Research Article

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