Compositional Analysis and Evaluation of Antioxidant Potential of Fruits of Withania somnifera Dunal

Authors

  • Ajay Pal Department of Chemistry & Biochemistry, CCS Haryana Agricultural University, Hisar-125004, India.
  • Bharat Bhushan "Scientist (Bio-Chemistry), Crop Processing Division, ICAR-CIPHET, Abohar, Punjab"
  • Vinod Saharan Department of Molecular Biology and Biotechnology, Maharana Pratap University of Agriculture & Technology, Udaipur, India.
  • Ramandeep Singh Sidhu Department of Chemistry, Brijindra College, Patiala University, Patiala, India.

Keywords:

Antioxidants, Extraction, Ashwagandha, Free radicals, Herbs

Abstract

The present investigation was carried out on the fruits of Withania somnifera to study its proximate
composition and in-vitro antioxidant potential. The fruits were found to have 4.67% crude protein, 2.23%
crude fat, 1.16% ash, 6.94% crude fiber and 13.39% total sugar on fresh weight basis. Highest total
phenolic content (TPC) and flavonoids were found in methanol extract (42.40; 4.76?g/mg extract) while
hexane fraction for TPC (3.07 ?g/ml extract) and water fraction for flavonoid (0.39?g/ml) were found
unsuitable for extraction. Methanolic extract showed highest hydroxyl radical activity (IC50= 3.48 mg),
total reducing power (IC50= 0.46 mg), metal chelation capacity (0.29 mg) and DPPH radical scavenging
capacity (0.31 mg).IC50 value of hexane extract for DPPH radical and chloroform extract for total reducing
power were the highest. In conclusion, the overall highest antioxidant potential of methanolic extract
can be attributed to TPC and flavonoids contents present in the extract.

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Published

2018-09-07

Issue

Vol. 1 No. 1&2 (2018): Journal of Advanced Research in BioChemistry and Pharmacology

Section

Research Article

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