Mitotic Index and its Role in Squamous Cell Carcinoma of Oral Cavity
Abstract
Background: Oral cavity carcinomas include lips, buccal mucosa, gingiva, anterior two-third of tongue, floor of the mouth and hard palate. In cancer, atypical mitosis and its increase indicates genetic damage in tumours and loss of controlled proliferation. Therefore, identification and quantification of mitosis forms an important part of the histological grading systems.
Materials and Methods: The sample of the study included 37 H&E stained slides diagnosed for various grades of Oral Squamous Cell Carcinoma from the archives of the Department of Pathology, Sharda University, School of Medical Science and Research, Uttar Pradesh, India. The sections were analysed for mitosis under a light microscope, under 40x.
Results: Out of the 37 cases, four cases were Carcinoma in Situ, 16 cases were well-differentiated squamous cell carcinoma, 12 cases were moderately differentiated squamous cell carcinoma and five cases were poorly differentiated squamous cell carcinoma. It was found that the mean mitotic count in males was 4.17 and females was 2.88. This was statistically insignificant. The mean mitotic count of buccal mucosa and tongue was 3.42, 3.49 respectively, which was insignificant. The mean mitotic count in carcinoma in situ, well differentiated, moderately differentiated and poorly differentiated was 5.00±6.880, 3.31±3.281, 4.50±4.661, 3.40±3.782 respectively, which came out to be statistically insignificant.
Conclusion: Methods of detecting tumour type in oral cavity and prognostication should be supplemented with Ki67 or other immunohistochemistry markers as has been done in luminal classification of breast carcinoma.
How to cite this article:
Chauhan I, Wonhyeong K, Trisal M. Mitotic Index and its Role in Squamous Cell Carcinoma of Oral Cavity. Rec Adv Path Lab Med. 2021;7(3&4):19-22.
DOI: https://doi.org/10.24321/2454.8642.202108
References
Rivera C. Essentials of oral cancer. Int J Clin Exp Pathol. 2015;8(9):11884-94. [Google Scholar]
Khandekar SP, Bagdey PS, Tiwari RR. Oral cancer and some epidemiological factors: A hospital based study. Indian J Community Med. 2006;31(3):157-9. [Google Scholar]
Speight PM, Farthing PM, Bouquot JE. The pathology of oral cancer and precancer. Curr Diagn Pathol. 1996;3:165–76.[Google Scholar]
Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2018 Nov;68:394-424. [PubMed] [Google Scholar]
Saran R, Tiwari RK, Reddy PP, Ahuja YR. Risk assessment of oral cancer in patients with pre-cancerous states of the oral cavity using micronucleus test and challenge assay. Oral Oncol. 2008 Apr;44(4):354-60. [PubMed] [Google Scholar]
Liu X, Gao XL, Liang XH, Tang YL. The etiological spectrum of head and neck squamous cell carcinoma in young patients. Oncotarget. 2016 Oct;7(40):66226-38. [PubMed] [Google Scholar]
Dhanuthai K, Rojanawatsirivej S, Thosaporn W, Kintarak S, Subarnbhesaj A, Darling M, Kryshtalskyj E, Chiang CP, Shin HI, Choi SY, Lee SS, Aminishakib P. Oral cancer: A multicenter study. Med Oral Patol Oral Cir Bucal. 2018 Jan;23(1):e23-9. [PubMed] [Google Scholar]
Subashini V. Mitotic figures evaluation in oral squamous cell carcinoma using crystal violet and feulgen stains - A comparative study. Int J Orofac Bio. 2019 Oct;3(2):42-4. [Google Scholar]
Haschek WM, Rousseaux CG, Wallig MA, Bolon B, Ochoa R, editors. Haschek and Rousseaux’s handbook of toxicologic pathology. Academic Press; 2013 May 1. [Google Scholar]
Shah S, Kaur M. Biomarkers and chemopreventives in oral carcinogenesis and its prevention. J Oral Maxillofac Pathol. 2014 Jan;18(1):69-76. [PubMed] [Google Scholar]
Meyer JS, Alvarez C, Milikowski C, Olson N, Russo I, Russo J, Glass A, Zehnbauer BA, Lister K, Parwaresch R; Cooperative Breast Cancer Tissue Resource. Breast carcinoma malignancy grading by Bloom-Richardson system vs proliferation index: reproducibility of grade and advantages of proliferation index. Mod Pathol. 2005 Aug;18(8):1067-78. [PubMed] [Google Scholar]
Kapoor K, Puri A, Prakash A, Jazib Sharma G. Mitotic counting and its significance in histopathological grading of OSCC & oral epithelial dysplasia. Head talk. 2013;5(6):35-7.
Kamel OW, Franklin WA, Ringus JC, Meyer JS. Thymidine labeling index and Ki-67 growth fraction in lesions of the breast. Am J Pathol. 1989 Jan;134(1):107-13. [PubMed] [Google Scholar]
Cooper GM. The eukaryotic cell cycle. The cell: a molecular approach. 2nd edition. Sunderland (MA): Sinauer Associates; 2000. [Google Scholar]
Bologna-Molina, Ronell et al. Comparison of the value of PCNA and Ki-67 as markers of cell proliferation in ameloblastic tumors. Med Oral Patol Oral Cir Bucal. 2013 Mar;18(2):e174-9. [PubMed] [Google Scholar]
Chitra NS, Boaz K, Srikant N, Lewis AJ, Sneha KS. Pattern-Corrected Mitotic Activity Index (PMAI): A novel prognosticator of oral squamous cell carcinoma. Turk Patoloji Derg. 2020;36(1):31-8. [PubMed] [Google Scholar]
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