The CRISPR-Cas9 Revolution: A Biology and Beyond Game-Changer
Keywords:
CRISPR-Cas9, Gene Editing, Genetic Diseases, Agriculture, Biotechnology, Human Development, Disease Mechanisms, Precision Medicine, Ethical ConsiderationsAbstract
The discovery of the CRISPR-Cas9 system ignited a biological revolution,
providing researchers with a potent and precise gene editing tool.
This review article delves into the history of CRISPR-Cas9, its method
of action, and its numerous applications in biology, including genetic
disease treatment, agriculture, and biotechnology. The ethical concerns
and potential limitations of CRISPR-Cas9 technology are also addressed.
The essay finishes by emphasising CRISPR-Cas9’s enormous contribution
to biological research and its potential to change the future of medicine
and innovation.
The CRISPR-Cas9 system has transformed biology by giving researchers
with an unprecedented gene editing tool with broad applications.
This review article delves into the history of CRISPR-Cas9, its method
of action, and its numerous applications in biology, including genetic
disease treatment, agriculture, and biotechnology. The ethical
concerns and potential limitations of CRISPR-Cas9 technology are also
addressed. The article emphasises CRISPR-Cas9’s major contribution to
biological research and its potential to change the future of medicine
and innovation. Overall, this study provides a thorough summary of
CRISPR-Cas9, emphasising its transformative potential as well as the
ethical issues surrounding its use.
References
Jinek M, Chylinski K, Fonfara I, et al. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science. 2012;337(6096):816-821.
Barrangou R, Fremaux C, Deveau H, et al. CRISPR provides acquired resistance against viruses in prokaryotes. Science. 2007;315(5819):1709-1712.
Sander JD, Joung JK. CRISPR-Cas systems for editing, regulating and targeting genomes. Nat Biotechnol. 2014;32(4):347-355.
Hsu PD, Lander ES, Zhang F. Development and applications of CRISPR-Cas9 for genome engineering. Cell. 2014;157(6):1262-1278.
Rees HA, Liu DR. Base editing: precision chemistry on the genome and transcriptome of living cells. Nat Rev Genet. 2018;19(12):770-788.
Cong L, Ran FA, Cox D, et al. Multiplex genome engineering using CRISPR/Cas systems. Science. 2013;339(6121):819-823.
Liang P, Xu Y, Zhang X, et al. CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes. Protein Cell. 2015;6(5):363-372.
Ma H, Marti-Gutierrez N, Park SW, et al. Correction of a pathogenic gene mutation in human embryos. Nature. 2017;548(7668):413-419.
Liang P, Sun H, Sun Y, et al. Effective gene editing by high-fidelity base editor 2 in mouse zygotes. Protein Cell. 2017;8(8):601-611.
Zhang Y, Ge X, Yang F, et al. Comparison of non-canonical PAMs for CRISPR/Cas9-mediated DNA cleavage in human cells. Sci Rep. 2014;4:5405.