Protein–DNA Interaction: Detection Techniques and Protocol
Keywords:
DNA, ProteinAbstract
DNA-binding proteins control cellular processes and cell cycles like recombination, replication and record. This survey is expected to sum up the absolute most regularly utilised methods to decide DNA-protein cooperations. In vitro methods like foot printing tests, electrophoretic versatility shift measure, southwestern blotting, yeast one-cross breed
tests, phage show and vicinity ligation examine have been talked about. Rotine-DNA interactions are important in a variety of biological activities, including gene control, DNA replication, repair, transcription, recombination, and chromosomal DNA packaging. Proteins recognise a sequence by possessing a surface that is chemically complementary to the DNA’s, resulting in a succession of favourable electrostatic and van der Waals interactions between the protein and the base pairs.
How to cite this article:
Pooni N. Protein–DNA Interaction: Detection
Techniques and Protocol. Rec Trends Pharm Tech
IndI 2024 6(2): 1-4.
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