A Study of Antiobesity, Hypolipidemic and Anti-Atherogenic Activity of Poly Herbal Formulation against High Fat Diet-Induced Obesity in Albino Rats
Keywords:
Antiobesity, Atherogenic Index, Curcuma Longa, Emblica Officinalis, Gymnema sylvestre, Hypolipidemic, OrlistatAbstract
Obesity is one of the most common health problems and has become an epidemic on the global scale. Hyperlipidaemia is one of the greatest risk factors contributing to atherosclerosis and occurrence of coronary heart diseases from the first human civilization, research is going to find the drugs to treat obesity and its complications. Despite availability of many drugs in market to treat obesity, no single drug is ideal for treating all sorts of problems caused by obesity. So the research is going on finding perfect drug. In the present study was aimed to evaluate Antiobesity, Hypolipidemic and Anti-Atherogenic activity of polyherbal formulations fruit of Emblica officinalis, Rhizome of Curcuma Longa & Leaves of Gymnema sylvestre in high fat fed albino rats. Obesity was induced in wistar albino rats by feeding them with high fat diet for 25 days. Group-I served as normal control (1% Carboxy Methyl Cellulose (CMC)) and Group-II as obese control (1% CMC) fed on high fat diet, Group- III, IV were treated with various polyherbal formulations, Group-V served as positive standard (Orlistat 50 mg/kg body wt.). Hyperlipidaemia was induced by feeding animals with high fat diet per orally, consisting of coconut oil and Vanaspati ghee, daily ad libitum except normal control. The animals were treated for 14 days. At the end of the study, blood samples of the animals were sent for the estimation of the lipid profile and effects of test drug studied by comparing levels of the body weight, Total Cholesterol, Triglycerides, HDL, LDL, and Atherogenic index. The statistical significance between groups was analysed. There was a significant reduction in food intake, body weight, TC, TG, LDL and an increase in HDL levels in high fat diet fed rats treated Polyherbal formulation containing Emblica officinalis, Rhizome of Curcuma Longa & Leaves of Gymnema sylvestre as compared to the Positive Standard and Normal treated animals.
References
2. Ambasta SP. The Useful Plants of India. Council of Scientific and Industrial Research, New Delhi, 1986.
3. Medicinal Plants of India. India Council of Medical Research, New Delhi, 1987.
4. Indian medicinal plants: A sector study. Ethnobotany. 1997; 17: 11.
5. Bhalla NP, Sahu TR, Mishira GP et al. Traditional plant medicines of Sagar district, Madhya Pradesh. J Econ Taxon Bot 1982; 3: 23-32.
6. Tiwari P. Phytochemical and pharmacological properties of Gymnema sylvestre: an important medicinal plant. Hindawi Publishing Corporation BioMed Research International 2014; 5: 18.
7. Kokate CK, Purohit AP, Gokhle SB. Textbook of Pharmacognosy. Nirali Prakashan, Pune. 1996; 2: 210.
8. Kokate CK. Practical Pharmacognosy. Vallabh Prakashan, Delhi. 2001; 4: 123, 125.
9. Dutta AC. Botany for Degree Students. Oxford University Press, New Delhi. 1964; 1: 177-179.
10. Najafi S. Studies on Gymnema sylvestre - a medicinally important plant of the family Asclepiadaceae. Trakia Journal of Sciences 2011; 9(2): 26-32.
11. The Ayurvedic Pharmacopoeia of India, Part-I. Vol-I, The controller publication, Government of India, Ministry of Health & Family Welfare. 2001: 137-146.
12. Quality control methods for medicinal plant materials. World Health Organization, Geneva. 1998: 8-30.
13. Harborne JB. Phytochemical Methods. Chapman and Hall, New York. 1984.
14. Mukherji PK. Quality control of herbal drugs, an approach to evaluation of botanicals, business horizons, pharmaceutical publishers, New Delhi. 2003; 1: 492.
15. Wagner H, Blandt S, Zgainsky SM. Plant Drug Analysis. Sprigher Veralg Heidelberg. 1996; 170, 176.
16. Quality Control Method for medicinal plant material. The World Health Organization, 2002.
17. Sagar D, Nirzarini SN, Punam SD et al. A study of antihyperlipidaemic activity of polyherbal formulation using various experimental animal models. Inventi Rapid: Ethnopharmacology 2011; 2(1): 1-4.
18. Trease GE, Evans WC. A textbook of Pharmacognosy. Bailliere Tindall Publication. London. 1978; 11: 530.
19. Maruthappan V, Sakthii S. Hypolipidemic activity of Haritaki (Terminalia chebula) in atherogenic diet induced hyperlipidaemic rats. J Adv Pharm Tech Res. 2010; 1(2): 229-235.
20. Saravanan R, Pari L. Antihyperlipidemic and antiperoxidative effect of Diasulin. A polyherbal formulation in alloxan induced hyperglycaemic rats. Complement Altern Med 2005; 5: 14.
21. Girish C, Koner BC, Jayanthi S et al. Hepatoprotective activity of six polyherbal formulations in paracetamol induced liver toxicity in mice. Indian Journal of Medical Research 2009; 129(5): 569-578.
22. Satoskar RS, Bhandarkar SD, Rege NN. Appetite stimulants, digestants, anti flatulents, appetite suppressants and hypolipidemic agents. Pharmacology and Pharmacotherapeutics. Popular Prakashan Pvt. Ltd., Mumbai. 2011; 22: 571-86.
23. Harsh Mohan. The Blood Vessels and Lymphatics: The Textbook of Pathology. Jaypee Brothers Medical Publisher (P) Ltd., Mumbai. 2000; 4: 252-254.
24. Mishra M, Pathak UN, Khan AB. Emblica officinalis Gaertn and serum cholesterol level in experimental rabbits. Br J Exp Pathol 1981; 62(5): 526-528.
25. World Health Organization. Obesity: preventing and managing the global epidemic. Report of a WHO consultation. WHO technical Report series 2000; 894(1- 12): 1-253.
