Larvicidal and Antibacterial Activities of Methanol Extract of Acacia polyacantha Willd
Keywords:
Acacia polycantha, Biological activity, Larvicidal activityAbstract
There is an immense need to develop an alternative antimicrobial source for treatment of several infectious diseases and restricting their vectors. So, evaluation of phytochemical constituents, antimicrobial and larvicidal activity of Acacia polyacantha bark methanol extract was undertaken in the present study. Proximate phytochemical analysis was performed according to standard methods using conventional protocols. Biological activates of the extract was conducted accordingly. The antibacterial screening was carried out against several bacterial pathogens. The larvicidal activity was evaluated by using Culex quinquefasciatus larva. The results revealed the presence of tannins, saponins, steroids, flavonoids, alkaloids and coumarins. The extract exhibited significantly higher diameter of inhibition zone against Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa compared to amoxicillin, except with Staphylococcus aureus. The maximum diameter of inhibition was recorded as 19±0.05 mm against E. coli. The extract showed a significant dose dependent effect with mortality up to 100% at 500 ?L. The LC50 obtained was 26.55 ?L and 27.11 ?L at 24 h and 48 h, respectively, while the LC90 was 318.5 ?L and 328.1 ?L at 24 h and 48 h, respectively. The results obtained in the present study suggest that the methanol extract of A. polyacantha revealed its potential anti-bacterial and larvicidal effects and clarifies its widely medicinal uses.
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