Establishment of an Efficient Protocol for Micropropagation of an Important Food Crop Finger Millet (Eleusine coracana) in Jharkhand Condition

Abstract

Eleusine coracana is an annual plant that is commonly cultivated in dry regions of Asia and Africa as a cereal. Finger millet seeds are harvested as explants. After that, seeds are sterilised using different concentrations of bavistin and HgCl2 (under LAF). Entire colonies of undifferentiated cells start to proliferate when the seeds are cultured on MS medium. It is possible to modify the undifferentiated cells using techniques like DNA extraction and transgene and selectable marker transformation. Growth hormones are introduced to the medium, and after 10 minutes, the cell responds best at 0.1% HgCl2, or 81.25%. To determine the most effective auxin type and concentration, the effects of several auxin concentrations (1 mg/l, 2 mg/l, 3 mg/l, and 4 mg/l) on callus and plant regeneration were assessed. The highest callus growth was found at 4 mg/l 2,4-D, or 72.22%. In 80% of the cultures, all of the auxin at concentrations of 3.0 and 4.0 mg/l caused callus. The greatest shoot development was seen at 2 mg/l BAP, or 81.25%.