Journal of Advanced Research in Biochemistry and Pharmacology

Biochemical Formulations: Integrating Technology and Healthcare

Abdul Waheed — 2024-10-09

In the evolving landscape of healthcare, the integration of technology with biochemical formulations represents a paradigm shift towards personalized and precision medicine. This abstract explores the intersection of technology and healthcare through the lens of biochemical formulations, emphasizing their role in diagnostics, treatment, and patient care. Biochemical formulations encompass a wide array of pharmaceuticals, from traditional small molecules to biologics and nanomedicines, each tailored to interact with specific biological targets or pathways. The integration of advanced technologies, such as artificial intelligence, genomic profiling, and targeted drug delivery systems, enhances the efficacy and safety of biochemical formulations while optimizing treatment outcomes. Moreover, these innovations facilitate real-time monitoring of biochemical markers, enabling clinicians to tailor therapies based on individual patient characteristics and disease profiles.

How to cite this article:
Waheed A, Khan N, Gul H, et al. Biochemical
Formulations: Integrating Technology and
Healthcare. J Adv Res Biochem Pharma 2024;
7(1): 6-12.

Antibacterial and Cytotoxic Properties of Terminalia chebula Fruits

Akansha Mukherji — 2024-10-09

Terminalia chebula fruits are recognized for their potent antibacterial and cytotoxic properties, offering significant potential as sources of bioactive compounds for therapeutic use. These fruits exhibit robust antibacterial activity against a wide array of pathogenic bacteria, encompassing both Gram-positive and Gram-negative species. The efficacy is attributed to their rich composition of tannins, flavonoids, and phenolic acids, which disrupt bacterial cell walls, inhibit essential enzymes, and chelate vital metal ions crucial for bacterial growth. This multifaceted approach not only curtails bacterial proliferation but also mitigates the risk of antibiotic resistance development. Moreover, Terminalia chebula enhances the effectiveness of conventional antibiotics through synergistic interactions, promising more efficacious treatments for bacterial infections. On the cytotoxic front, Terminalia chebula demonstrates potent activity against diverse cancer cell lines, including those associated with breast, colon, and liver cancers. Mechanistically, the fruits induce apoptosis via caspase activation and mitochondrial pathways, facilitated by key compounds such as chebulagic acid, chebulinic acid, and ellagic acid.

How to cite this article:
Mukherji A, Gosh D. Antibacterial and Cytotoxic
Properties of Terminalia chebula Fruits. J Adv Res
Biochem Pharma 2024; 7(1): 1-5.

Melatonin and Its Impact on Circadian Rhythms and Sleep

Agung — 2024-10-09

Melatonin, synthesized primarily in the pineal gland, acts as a biological signal synchronized to the day-night cycle, influencing various physiological processes through interactions with MT1 and MT2 receptors. By modulating clock gene expression in the Suprachiasmatic Nucleus (SCN), melatonin plays a pivotal role in maintaining robust circadian rhythms governing sleep-wake cycles, hormone secretion, and metabolic functions. Beyond its chronobiotic effects, melatonin
enhances sleep quality by promoting sleep onset and optimizing sleep architecture, including Rapid Eye Movement (REM) and Slow-Wave Sleep (SWS) stages. Clinical applications of melatonin extend to managing sleep disorders, circadian rhythm disturbances, and conditions associated with oxidative stress and neurodegeneration. Understanding melatonin’s mechanisms of action provides insights into its therapeutic potential and underscores its role in optimizing sleep health and overall well-being.

How to cite this article:
Agung, Bayu, Fadhlan. Melatonin and Its Impact
on Circadian Rhythms and Sleep. J Adv Res
Biochem Pharma 2024; 7(1): 200-00.

Plant-Based Anti-inflammatory Compounds: Current Research and Applications

Abdul Rahman — 2024-10-09

Quantitative Analysis of Antioxidant Biomarkers in Cucumis Melo Var. Agrestis Leaves Using a Validated Hptlc Technique

Varun Naik — 2024-10-09

Background: This study focused on using the HPTLC method for the simultaneous estimation of natural antioxidant markers, specifically rutin, gallic acid, and quercetin, from the plant extract of Cucumis melo var. agrestis (CME).
Aims and Objectives: The primary objective of this research was to extract and simultaneously quantify the three antioxidant markers— rutin, gallic acid, and quercetin—from CME using normal phase HPTLC.
Materials and Methods: Compounds were separated on TLC aluminum plates precoated with silica 60 F 260, followed by the detection of rutin, gallic acid, and quercetin. Densitometric scanning at 250 nm was performed using a Camag TLC scanner 3 equipped with winCATS software. This method was further validated for linearity, precision, accuracy, and sensitivity according to ICH guidelines.
Results: The calibration plots showed a strong linear relationship with R2 values of 0.982, 0.9892, and 0.9860 for rutin, gallic acid, and quercetin, respectively. Accuracy was assessed through recovery studies at three different levels, yielding an average recovery rate between 95% and 98% for all three markers.
Conclusion: The developed HPTLC method is suitable for detecting antioxidant markers in botanicals and herbal formulations.

How to cite this article:
Quantitative Analysis of Antioxidant Biomarkers
in Cucumis Melo Var. Agrestis Leaves Using a
Validated Hptlc Technique. J Adv Res Biochem
Pharma 2024; 7(1): 30-0.