Water Extract of Three Aromatic Plants Mixture Ameliorates Paracetamol-Induced Renal-Hepato Damage in Male Albino Rats

Authors

  • Hatil Hashim EL-Kamali Department of Botany, Faculty of Science and Technology, Omdurman Islamic University, Omdurman, Sudan.
  • Nadia Nour El-Din Abd-AL-Gadir Department of Botany, Faculty of Science and Technology, Omdurman Islamic University, Omdurman, Sudan.
  • Manal Mohammed Ramadan Department of Chemistry of Flavour and Aroma, National Research Centre, Dokki, Giza, Egypt.
  • Kadry Z. Ghanem Department of Clinical Nutrition, Faculty of Applied Medical Science, Jazan University, KSA.
  • Abdel Razik H. Farrag Department of Pathology, National Research Centre, Dokki, Giza, Egypt.

Keywords:

Xylopia aethiopica, Coriandrum sativum, Anethum graveolens, Hepato-Renal Protective Activity, Rats

Abstract

Background and Objectives: This study examined the effects of fruit water extracts of mixture from selected three plants (Xylopia aethiopica, Coriandrum sativum and Anethum graveolens) on some indices of liver and kidney function tests in the male albino rats, and also to evaluate the antioxidant power of its water extract and the protective effect against paracetamol-induced hepatic and renal toxicity in male albino rats.

Materials and Methods: Three groups of rats were used (control, paracetamol-treated and protected group), which were supplemented with mixture water extract for four weeks followed by intraperitoneal injection of paracetamol. Levels of ALT, AST, ALP, gamma-glutamyl transferase, CAT, GPx, serum direct bilirubin, serum total bilirubin, serum albumin, serum total globin, total serum urea and serum creatinine, as well as histopathological changes in the liver and kidney were investigated. Quantitative determination of the total phenolic content (TPC) was performed using the Folin-Ciocalteu method, quantitative determination of antioxidant activity was performed according to the ?-carotene bleaching method and Diphenylpicrylhydrazyl (DPPH) free radical scavenging assay. The experiment was conducted for two weeks. Statistical analysis was carried out by analysis of variance.

Results: Oral administration of paracetamol recorded significant decrease in TAC, CAT and GPx compared to normal control rats whereas rats supplemented with water extract and then intoxicated with paracetamol showed a significant increase in TAC, CAT and GPx levels compared with paracetamol intoxicated rats, and compared to normal control. AST, ALT, ALP, GGT, total bilirubin, direct bilirubin and indirect bilirubin levels of rats treated with paracetamol were quite higher than that of control group whereas the rats treated with mixture extract and paracetamol had significant reduction when compared with the paracetamol group. Paracetamol induced significant increase in the concentration of plasma urea and creatinine in intoxicated rats as compared to normal control rats, whreas there was no significant difference between mixtures extract supplemented rats and protected rats as compared with control.

Conclusion: The aqueous extract mixture dose dependably offered potential renal-hepato protection from paracetamol induced hepatic damage, normalizing biochemical parameters in rats. 

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Published

2019-04-15

Issue

Vol. 2 No. 1 (2019): Journal of Advanced Research in BioChemistry and Pharmacology

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Research Article

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