Detection of Jatropha Mosaic Indian Virus (JMIV) on Jatropha Plant (Jatropha Curcas)

Authors

  • Abdullahi Mohammad National Biotechnology Development Agency, Bioresources Development Centre, P. M. B. 2140 Katsina, Katsina State, Nigeria.
  • Bello I National Biotechnology Development Agency, Bioresources Development Centre, P. M. B. 2140 Katsina, Katsina State, Nigeria
  • Ahmad m National Biotechnology Development Agency, Bioresources Development Centre, P. M. B. 2140 Katsina, Katsina State, Nigeria
  • Abdurrahman s a National Biotechnology Development Agency, Bioresources Development Centre, P. M. B. 2140 Katsina, Katsina State, Nigeria
  • Yusuf m b National Biotechnology Development Agency, Bioresources Development Centre, P. M. B. 2140 Katsina, Katsina State, Nigeria.
  • Idris m National Biotechnology Development Agency, Bioresources Development Centre, P. M. B. 2140 Katsina, Katsina State, Nigeria.
  • Maruthi M.N Natural Resource Institute (NRI) University of Greenwich at Medway Central Avenue Chatham Maritime Kent ME4 4TB, United Kingdom )
  • Seal S.E Natural Resource Institute (NRI) University of Greenwich at Medway Central Avenue Chatham Maritime Kent ME4 4TB, United Kingdom )
  • Garga MA National Biotechnology Development Agency, Bioresources Development Centre, P. M. B. 2140 Katsina, Katsina State, Nigeria.

Keywords:

Core Coat Protein, Polymerase Chain Reaction, JMIV, Ultra Violet Light

Abstract

Jatropha plant is a major crop for biofuel production in countries such as India. The recent increase in the cultivation of Jatropha has resulted in the manifestation of pest and disease attacks on the crop, including jatropha mosaic Indian virus (JMIV) which is a member of the genus Begomovirus (family Geminiviridae). Jatropha leaves were collected from Bangalore, South India showing mosaic symptoms, malformation of leaves and chloric specks. Total DNA was extracted from infected leaves and the extracted DNA was subjected to PCR to amplify JMIV DNA using degenerate primers and subsequently with begomovirus- specific primers for both the DNA-A (JMIV-A 970F and JMIV-A 970R) and DNA-B (JMIV-B 970F and JMIV-B 970R). The PCR products obtained with degerate primers were ~ 530bp which further confirmed the association of a begomovirus with jatropha. Furthermore, PCR products in the amplification of both full-length DNA-A and DNA-B components of JMIV were successful for the first time which produces ~ 2.8 kb on both the genome.

References

1. Joachim H. Physic nut; Jatropha curcas L. International Plant Genetics Resources Institute, Via Delle Sette Chiese 142, 00145 Rome, Italy. 1996.
2. Reinhard KH. Use of Jatropha curcas oil as row material and fuel; an integrated approach to create income and supply energy for rural development. Jatropha Project, West Africa. 2000.
3. Olaniyi A. Jatropha promotion in Nigeria: The green gold, Centre for Jatropha Promotion & Biofuel, Nigeria. 2003.
4. Brown JK, Idiris AM, Bird J. Jatropha gassipiifolia from Puerto Rico. Journal of Phytopathology 1999; 90: SI 122. 5. Narayana HA, Rangaswamy KT, Shankarppa KS et al. Distinct Begomoviruses Closely Related to Cassava Mosaic Viruses Cause Indian Jatropha Mosaic Disease. International Journal of Virology 2007; 3: 1-11.
6. Paximadis M, Rey MEC. Genomic organisation of tomato leaf curl Zimbabwe virus, a new distinct monopartite Begomovirus associated with sub genomic defective DNA molecule. Journal of Gen Virology 2001; 82: 3091- 3097. 7. Smith CJ. The Geminivirus group: Biochemistry and molecular biology of plant- pathology interaction. Research Studies Press Ltd., 58D Station Road Letchworth, SG6 3BE, England. 1991. 50-51.
8. Lazarowitz SG. Gemini viruses, genome structure and function. Critical Review of Plant Science 1992a; 11: 327-349.
9. Gafni Y, Epel BL. The role of host and viral protein in intra-cellular trafficking of Gemini viruses. Physiological and Molecular Plant Pathology 2002; 60: 231-241.
10. Seal SE, Vanden Bosch F, Teger MJ. Factors infecting influencing Begomoviruses Evolution and their increasing global significant, implication for Sustainable Control. Critical Review in Plant Science 2006a; 25: 23-26. 11. Narayana HA, Rangawamy KT, Ranghasvandra KS et al. Natural occurrence of a mosaic virus disease in India. Current Science 2006; 91: 584-586.
12. Cuellar ME, Morales FJ. La mosca blanca Bemisia tabaci (Gennadius) como plaga y vectora de virus en fríjol común (Phaseolus vulgaris L.). Revista Colombiana de Entomología 2006; 32: 1-9.
13. Lashkowitz D, Gazit S. Whitefly (Bamisia tabaci) genome project: analysis of sequenced clone from egg, instar and adult (viruliferous and non-viruferous) cDNA libraries. BMC Genomics 2006; 7: 79.
14. Maruthi MN, Colvin J, Seal SE, et al. Co-adaptation between cassava mosaic geminiviruses and their local vector population. Virus Research 2002; 86: 71-85.
15. Lodhi MA, YEG, Weeden NF, et al. A simple and efficient method for DNA extraction from grapevine cultivars and vitis species. Plant Molecular Biology 1994; 12: 6-13.
16. Denge D, McGrath PF, Robinson DJ, et al. Detection and differentiation of whitefly transmitted Gemini viruses in plants and vector insect by the polymerase reaction with degenerate primers. Annual Apply Biology 2009; 125: 327-336.
17. Raj SK, Snehi SK, Kumar S et al. First molecular identification of a begomovirus in India that is closely related to cassava mosaic virus and causes mosaic and stunting of Jatropha curcas L. Australasian Plant Disease Note 2008; 3: 67-72.

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Published

2019-04-15

Issue

Vol. 2 No. 1 (2019): Journal of Advanced Research in BioChemistry and Pharmacology

Section

Research Article

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