Iron and Silver Contributions to Systemic Toxicity in Albino Rats In Vivo

Authors

  • Tugbobo O S Department of Science Technology, Federal Polytechnic, Ado-Ekiti, Nigeria.
  • Idowu K S Department of Science Technology, Federal Polytechnic, Ado-Ekiti, Nigeria.
  • Segun Michael Abegunde Department of Science Technology, Federal Polytechnic, Ado-Ekiti, Nigeria.
  • Odutuga A A Department of Chemical Sciences, Joseph Ayo Babalola University, Ikeji-Arakeji, Nigeria.

Keywords:

Iron sulphate, Phosphatase, Silver nitrate, Transferase, Toxicity

Abstract

Indiscriminate exposure of man to iron and silver toxicity through a long-term ingestion of contaminated food, utensils and water coupled with its health challenges has been widely reported. This study examined the effect of iron (ll) sulphate (2.5 mg/kg body weight) and silver nitrate (1.5 mg/kg body weight) fed to albino rats consecutively alone and simultaneously for two weeks (14-days). Control animals were fed with distilled water. Hematological and biochemical analyses as well as histopathological examination of kidney and liver tissues were monitored. The findings indicate that there was significant (P<0.05) decrease in the values of hematological parameters while the histopathology of rats fed with the metals showed grossly hemorrhagic cells riddled with lesion of varying degrees. Besides, there was significant (P<0.05) difference in the values obtained for alkaline phosphatase (ALP), aspartate aminotransferase (AST) and acid phosphatase (ACP) when compared with control. The results suggest development of microcytosis, hepatotoxicity and nephrotoxicity induced by iron sulphate and silver nitrate simultaneously.

References

1. Abegunde SM, Dada EB, Adelaja OI. Assessment
of heavy metals distribution and contamination in
atmospheric dust from major roads in Ado-Ekiti, Ekiti
State using pollution indices. International Journal
of New Technology and Research 2018; 4(4): 34-40.
2. Brautbar MD. Neurobehavioral changes, hypertension
and kidney diseases. JAMA 2005; 253: 530-4.
3. Clarkson TW, Woolf AD. Metal toxicity in central
nervous system. Int J Occup Med Environ Health 2001;
14(3): 312-20.
4. Das SK, Nair RC. Lipid peroxidation of normal and
sickled erythrocytes. Br J Heamatol 1980; 44(1): 87-92.
5. Griffin WT, Fahim MS. Histopathological method on
tissue atrophy. JAMA 2002; 103: 331-6.
6. Lambert T, Lane DA. Iron, arsenic and PAH in soil and
house dust. Environ. Health Perspect 2004; 112(1):
39-41.
7. Leonard A. Metals and their deleterious effects in the
body. Biochem Biophys Acta 1991; 1288: 179-88.
8. Nehez M, Lorenez R, Desi I. Simultaneous action
of cypermethrin with cadmium and lead on rat
chromosomes in subchronic administration. Ecotoxicol
Environ Saf 2000; 45: 55-60.
9. Preston RJ, Dean BS, Galloway S et al. Mammalian
hematologic and bone marrow assay. Mutat Res 1987;
189: 157-65.
10. Rietzman S, Frankel S. Calometric method for the
determination of aspartate amino transferase and
alanine amino transferase. Am J Clin Pathol 1957;
28: 56-63.
11. Schmidt SH, Schmidt G. Biochemical symptoms of rat
liver cytolysis. Am J Clin Pathol 1979; 45: 101-109.
12. Tugbobo OS, Oloyede OI, Adewale O. Protection by
garlic against lead-induced oxidative stress and genetic
birth defects. Journals of Oxidants and Antioxidants
Medical Sciences 2014; 3(1): 79-82.
13. Wright DJ, Leathwood PD, Plummer DT. Enzymes in
rat urine; acid and alkaline phosphatases. Enzymology
1972; 42(6): 317-327.

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Published

2018-12-26

Issue

Vol. 1 No. 3&4 (2018): Journal of Advanced Research in BioChemistry and Pharmacology

Section

Research Article

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