Production of Pectinase by Aspergillus niger Isolated from Different Sites of Kathmandu Valley and Compare the Activity of the Best Strain
Keywords:
Aspergillus niger, Pectinase, Orange Peel, MesophileAbstract
With a view to explore the feasibility of pectinase enzymes which have optimal efficiencies at different temperatures and pH which thereby helping to increase enzyme efficiency. The mesophilic temperature range of Aspergillus niger was isolated from different soils and water samples of Kathmandu valley by incubating these in Potato Dextrose Aga for 4–7 days at 28oC after a series of dilution. A. niger was screened for the production of pectinase by using orange peel as the substrate. The amount of pectinase enzyme was evaluated using the biuret method. The pectinase activity of a different sample of A. niger was evaluated using the DNS method, cup well agar and Sigma Aldrich protocol of pectinase activity analysis. The pectinase activity of the A. niger was 120 units as compared with standard pectinase activity 220 units, which shows it is a good producer of pectinase. Pectinase was subjected to varying conditions of pH and temperature. The optimum condition for the production of pectinase by A. niger is found to be pH 6.2 and temperature 50oC. Thus, obtained extracellular pectinase can be utilized for industrial processes using citrus peel as a substrate.
How to cite this article: Sagar KK, Swarnakar N, Dahal B. Production of Pectinase by Aspergillus niger Isolated from Different Sites of Kathmandu Valley and Compare the Activity of the Best Strain. J Adv Res Biochem Pharma 2019; 2(2): 1-13.
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