Different Growth Factors Supplement and Growth Response in Callus Culture of Rice (Oryza Sativa L.)

Authors

  • Vasukidevi Ramachandran Associate Professor, Department of Biomedical Engineering, Bharath Institute of Higher Education and research, 173, Agaram Road, Selaiyur, Chennai-600073, Tamil Nadu, India

Keywords:

MS medium, Callus, Supplemented, Embryoss

Abstract

Callusing response of rice IR – 20 (explants – seed embryos ) was assessed in MS- medium supplemented
with 2,4-D, kinetin, Choline chloride, Glycine and Choline chloride with glycine separately. It was assessed
by performing protein estimation, cell number counting and on the basis of fresh and dry weight of the
callus. The callusing response was high (80%) in MS-medium supplemented with choline chloride. The
protein content was more in MS-medium supplemented with glycine. The number of cells present in the
callus raised on MS-medium supplemented with 2,4,-D and Kinetin was higher as compared to other growth
factors supplemented media Somatic embryos were observed in the callus culture raised on MS+2,4-D and
kinetin. Different protein levels were measured in the callus culture raised in MS-medium supplemented
with 2,4-D, Kinetin, Choline chloride and Glycine.

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Published

2018-09-07

Issue

Vol. 1 No. 1&2 (2018): Journal of Advanced Research in BioChemistry and Pharmacology

Section

Short Article

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