Confirmation of Best Method for Detection of Alpha Amylase Activity of Cucumis Melo Var Agrestis (Wild Musk Melon)

In vitro Antidiabetic activity of Wild musk melon

Authors

  • Gopalasatheeskumar K Department of Pharmacology, KMCH College of Pharmacy, Kovai Estate, Kalapatti Road, Coimbatore-641048, Tamil Nadu, India (Affiliated to The Tamil Nadu Dr. M.G.R. Medical University).
  • Ariharasivakumar G Department of Pharmacology, KMCH College of Pharmacy, Kovai Estate, Kalapatti Road, Coimbatore-641048, Tamil Nadu, India (Affiliated to The Tamil Nadu Dr. M.G.R. Medical University).
  • Sengottuvel T Department of Pharmacology, KMCH College of Pharmacy, Kovai Estate, Kalapatti Road, Coimbatore-641048, Tamil Nadu, India (Affiliated to The Tamil Nadu Dr. M.G.R. Medical University).
  • Arulkumaran G Department of Pharmaceutics, KMCH College of Pharmacy, Kovai Estate, Kalapatti Road, Coimbatore-641048, Tamil Nadu, India (Affiliated to The Tamil Nadu Dr. M.G.R. Medical University).
  • Sanish Devan V Department of Pharmacology, KMCH College of Pharmacy, Kovai Estate, Kalapatti Road, Coimbatore-641048, Tamil Nadu, India (Affiliated to The Tamil Nadu Dr. M.G.R. Medical University).
  • Srividhya V Department of Pharmacology, KMCH College of Pharmacy, Kovai Estate, Kalapatti Road, Coimbatore-641048, Tamil Nadu, India (Affiliated to The Tamil Nadu Dr. M.G.R. Medical University).

Keywords:

Iodine-starch Color Assay, DNSA Color Assay, In Vitro Antidiabetic Activity

Abstract

Background: Alpha amylase inhibition study important in the study of antidiabetic potential. Various methods involved in detection of alpha amylase inhibition detection of best method are important in research. But there is no paper regarding these criteria therefore current research is focused on detection of best method for alpha amylase activity.

Aim and Objective: Aim of this research is to identify the best method for detection of alpha amylase activity. Objectives of this research are to extract the plant material Cucumis melo var agrestis and evaluate alpha amylase inhibitory activity.

Methods: alpha amylase inhibition activity by iodine-starch calorimetric method and DNSA calorimetric method.

Results: inhibitory alpha amylase activity by DNSA method IC50 of standard acarbose was 372.04?g/ ml and HALEC was 445.30?g/ ml. inhibitory alpha amylase activity by DNSA method IC50 of standard acarbose was 129.46 ?g/ ml and HALEC was 153.31 ?g/ ml.

Conclusion: From the result concluded that HALEC has anti-diabetic potential and starch-iodine calorimetric method was best method for detection of alpha amylase inhibitory activity.

References

1. Bailey JE, Ollis DF. Biochemical Engineering Fundamentals, 2nd Ed., Chapter 3, McGraw-Hill, 1986.
2. Standard SKB method to determine enzyme activity: Cereal Chem, 16, 712, 1939.
3. Gopalasatheeskumar K. Significant Role of Soxhlet Extraction Process in Phytochemical Research. MJPMS 2018; 7(1):43-47.
4. Xiao Z, Storms R, Tsang A. A quantitative starch iodine method for measuring alpha-amylase and glucoamylase activities. Anal Biochem 2006; 351(1): 146-148.
5. Ganapaty S, Nandeesh R, Veerapur VP et al. Invivo and Invitro Anti-Diabetic Effects of Madhuca indica Roxb., in Alloxan-Induced Diabetic rats. IJAPBC 2013; 2(2): 282-290.
6. Miller Gl. Use of dinitrosalicylicacid reagent for determination of reducing sugar. Anal Chem 1959; 31: 426-428. 7. Sridhar SM, Pavithra TK, Ashok Kumar BS et al. Alpha Amylase Inhibition Activity of Poly Herbal Formulation (D-Scale). IJRPB 2015; 2(6): 8-10.

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Published

2019-06-01

Issue

Vol. 2 No. 1 (2019): Journal of Advanced Research in BioChemistry and Pharmacology

Section

Short Article

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