CRISPR Technology in Drug Discovery: Revolutionizing Target Identification and Therapeutic Development
Abstract
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technology has emerged as a revolutionary tool in the field of drug discovery, providing researchers with unprecedented precision and efficiency in gene editing. In this article we explore the applications of CRISPR technology in drug discovery, with a focus on target identification and therapeutic development. We delve into the underlying mechanisms of CRISPR, its evolution, and the diverse CRISPR-based methodologies employed in drug discovery pipelines. The paper also addresses the challenges and ethical considerations associated with CRISPR applications in the pharmaceutical industry.
How to cite this article:
Reddy P. CRISPR Technology in Drug Discovery: Revolutionizing Target Identification and Therapeutic Development. Int J Adv Res Med Chem 2023; 5(2): 6-9
References
Doudna, Jennifer A., and Samuel H. Sternberg. 2017. A Crack in Creation: Gene Editing and the Unthinkable Power to Control Evolution. Boston: Houghton Mifflin Harcourt.
Jinek, Martin, et al. 2012. “A Programmable Dual- RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity.” Science 337 (6096): 816–21.
National Academies of Sciences, Engineering, and Medicine. 2017. Human Genome Editing: Science, Ethics, and Governance. Washington, DC: The National
Academies Press.
Cong, Le, et al. 2013. “Multiplex Genome Engineering Using CRISPR/Cas Systems.” Science 339 (6121): 819– 23.
Baltimore, David, et al. 2015. “A Prudent Path Forward for Genomic Engineering and Germline Gene Modification.” Science 348 (6230): 36–38.
Hsu, Patrick D., Eric S. Lander, and Feng Zhang. 2014. “Development and Applications of CRISPR-Cas9 for Genome Engineering.” Cell 157 (6): 1262–78.
National Institutes of Health. 2020. “Guidelines for Research Involving Recombinant or Synthetic Nucleic Acid Molecules.” Accessed January 5, 2023. https:// osp.od.nih.gov/biotechnology/nih-guidelines/.
Charpentier, Emmanuelle, and Jennifer A. Doudna. 2013. “Biotechnology: Rewriting a Genome.” Nature 495 (7439): 50–51.
Callaway, Ewen. 2015. “CRISPR Gene-Editing Tested in a Person for the First Time.” Nature News, November 15. https://www.nature.com/news/crispr-gene-editingtested- in-a-person-for-the-first-time-1.18852.
National Institute of Allergy and Infectious Diseases. 2018. “NIH Statement on He Jiankui’s Claim of Gene-Edited Babies in China.” Accessed January 5, 2023. https://www.niaid.nih.gov/news-events/nihstatement- he-jiankuis-claim-gene-edited-babies-china.
Ishino, Y., et al. 1987. “Nucleotide Sequence of the Iap Gene, Responsible for Alkaline Phosphatase Isozyme Conversion in Escherichia Coli, and Identification of the Gene Product.” Journal of Bacteriology 169 (12): 5429–33.
Berg, Paul, et al. 1974. “Summary Statement of the Asilomar Conference on Recombinant DNA Molecules.” Proceedings of the National Academy of Sciences 71 (12): 4821–23.
